Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) |
AAVcc47, Male |
Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells. |
liver |
Editing Efficiency |
15000001027 |
|
SaCas9-Lagor |
|
|
|
AAVcc47-Ai9-sgRNA1 + sgRNA2; AAVcc47-CMV-SaCas9; |
|
Mouse |
B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J |
|
intravenous |
liver and heart |
1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector |
once |
|
200uL |
4 weeks |
SaCas9 protein and sgRNAs expressed from AAV |
|
|
|
Measured Values |
Samples Size: | 3 |
|
Mean |
6.95  |
1 |
7.34656881960435  |
2 |
6.65631074773692  |
3 |
6.85436293266559  |