AAVcc47-Ai9-sgRNA1 + sgRNA2 | SCGE Toolkit

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Summary Projects & Experiments

SCGE ID	14000000049
Name	AAVcc47-Ai9-sgRNA1 + sgRNA2
Description	AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus
Type	Viral Vector
Subtype	AAV

Source	Asokan Lab

Genome Serotype	AAV2
Capsid Serotype	AAV9
Capsid Variant	452-GVSLGGG-458
Stock/Catalog/RRID	AAVcc47-Ai9-sgRNA1 + sgRNA2

Annotated Map	pLH9-GVSLGGG.gb
Titer Method	qPCR

Publication Title
Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing. NCBI

This Vector/Format: AAVcc47-Ai9-sgRNA1 + sgRNA2 is being used ...

Project	Initiative	Contact PI
Evolving High Potency AAV Vectors for Neuromuscular Genome Editing Experiments Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter)	Delivery Systems	Aravind Asokan, PhD (Duke University)