Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector. |
AAV9, Male |
Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells. |
liver |
Editing Efficiency |
15000001033 |
|
SaCas9-Lagor |
|
|
|
AAV9-CMV-SaCas9; AAV9_pTR_self comp 2xU6-Ai9 guides; |
|
Mouse |
B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J |
|
intravenous |
liver and heart |
2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector |
once |
|
200uL |
4 weeks |
SaCas9 protein and sgRNAs expressed from AAV |
|
|
|
Measured Values |
Samples Size: | 2 |
|
Mean |
6.91  |
1 |
6.42643209183603  |
2 |
7.39562181140885  |