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Arm 3: Negative Control Saline, Males | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
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Experiment Record Detail
Experiment
Independent validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain
Experiment Condition
Arm 3: Negative Control Saline, Males
Assay Description:
Qualitative assesment of tdTomato-negative cells throughout the entire brain
Tissue Measured:
brain
Measurement Type:
Editing Efficiency
Record ID
15000001462
Editor
Delivery System
Delivery System Subtype
Guide
Vector
Model Species
Mouse
Model Name
B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J
Application Method
Application Site
brain
Dosage
Injection Frequency
once
Injection Rate
Injection Volume
5 microliters/gram
Days post injection
4 weeks
Editor Format
none
Antidote Id
Antidote Description
Measured Values
Samples Size:
3
Replicate
Result (Editing Efficiency)
Measurement Units:
Signal
Mean
3 of 3 absent 
1
absent 
2
absent 
3
absent 
Experiment Wide Protocols
Title
Description
File Download
SCGE ID
Heaney_SATC Tissue Processing, Imaging and Analysis
Procedure for tissue preparation, imaging and analysis.
Heaney_SATC_Tissue_Processing_Imaging_and_Analysis.pdf
21000000025