Development System Independent validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain | SCGE Toolkit Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing. Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 reporter mice
Validation Experiment: Independent validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain
PI:
Jason D Heaney, PhD
Description: Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing. Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 reporter mice
Delivery Assays:
Identification of of GFP-positive nuclei (green)
Editing Assay:
Qualitative assesment of tdTomato-negative cells throughout the entire brain
Quantitative assessment of tdTomato-positive cells per DAPI-stained nucleus in a specific brain region
Qualitative assesment of prevalence of tdTomato-positvie cells throughout the entire brain
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Results
SCGE Toolkit downloaded on: 2024/10/09 22:51:52; Please cite the Somatic Cell Genome Editing Consortium Toolkit NIH HG010423 when using publicly accessible data in formal presentation or publication. SCGE Experment ID: 18000000049. PI:
Jason D Heaney PhD