Experiment Validating Traffic Light Reporter 2 (TLR2) Mouse Model in Heterozygous Blastocysts using SaCas9 Experiment Condition SaC9-g2 Assay Description: Reporter activation by NHEJ is calculated as the percent of blasts that express tagRFP out of the total blasts imaged. Approximately one third of NHEJ events are detected. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000003460 Editor sNLS-SaCas9-sNLS Nuclease Delivery System Delivery System Subtype Guide SaC9-g2 Vector Model Species Mouse Model Name TLR-2 Heterozygous Blastocyst Application Method Electroporation Application Site Dosage Injection Frequency Injection Rate Injection Volume Days post injection 96 hours Editor Format RNP Antidote Id Antidote Description Measured Values Samples Size: 0   Replicate Result (Editing Efficiency) Measurement Units: % of blasts with RFP activation Mean 10  1 10.0  Measurement Units: % of blasts with edits Mean 36  1 36.0  Measurement Units: Signal Mean # blasts analyzed  1 67 blasts analyzed for edits  Mean # blasts analyzed  1 67 blasts analyzed for RFP