Validating Traffic Light Reporter 2 (TLR2) Mouse Model in Heterozygous Blastocysts using SaCas9 |
SaC9-g3 |
Reporter activation by NHEJ is calculated as the percent of blasts that express tagRFP out of the total blasts imaged. Approximately one third of NHEJ events are detected. |
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Editing Efficiency |
15000003461 |
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sNLS-SaCas9-sNLS Nuclease |
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SaC9-g3 |
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Mouse |
TLR-2 Heterozygous Blastocyst |
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Electroporation |
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96 hours |
RNP |
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Measured Values |
Samples Size: | 0 |
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Mean |
13  |
1 |
13.0  |
Mean |
74  |
1 |
74.0  |
Mean |
# blasts analyzed  |
1 |
55 blasts analyzed for edits  |
Mean |
# blasts analyzed  |
1 |
55 blasts analyzed for RFP  |