Experiment Validating Gene Editing Reporter 14 (GER14) Mouse Model in Heterozygous Blastocysts Experiment Condition Cre Assay Description: Reporter activation by NHEJ is calculated as the percent of blasts that express Katushka2S (RFP) out of the total blasts imaged. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000003593 Editor CleanCap® Cre mRNA Delivery System Delivery System Subtype Guide Vector Model Species Mouse Model Name GER14 Heterozygous Blastocyst Application Method Electroporation Application Site Dosage Injection Frequency Injection Rate Injection Volume Days post injection 96 hours Editor Format mRNA Antidote Id Antidote Description Measured Values Samples Size: 0   Replicate Result (Editing Efficiency) Measurement Units: % of blasts with RFP activation Mean 84.78  1 84.78  Measurement Units: % of blasts with edits Mean 97.44  1 97.44  Measurement Units: Signal Mean # blasts analyzed  1 46 blasts for RFP  Mean # blasts analyzed  1 39 blasts for edits

Cre Validation of Gene Editing Reporter 14 (GER14) Mouse Model in Heterozygous Blastocysts Zygotes were electroporated with Cre mRNA and cultured 96 hours to blastocyst stage. Blastocysts were imaged for Katushka2S (RFP) fluorescence and scored for fluorescence signal.