Experiment Inhibitory effect of Cas9 RNP alone on S10- or FSDS315-mediated GFP protein delivery to HeLa cells Experiment Condition S10 + Cas9 RNP Assay Description: Flow Cytometry Tissue Measured: Measurement Type: Delivery Efficiency Record ID 15000002987 Editor SpCas9 (Feldan Therapeutics) Delivery System S10 Delivery System Subtype Amphiphilic peptide Guide Cas9-CFTR-R553X Vector Model Species Human Model Name HeLa Application Method Application Site Dosage GFP protein (10μM) + Shuttle peptide (10μM) + Cas9 RNP (2.5 μM Cas9 and 2 μM gRNA) Injection Frequency Injection Rate Injection Volume Days post injection Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Delivery Efficiency) Measurement Units: % of fluorescent cells Mean 3.48  1 1.68  2 1.03  3 7.19  4 4.02