Contact SCGE




SCGE Consortium Home   |  About SCGE Toolkit   |  Contact Us   |  Citing the Toolkit   |  License  
ExperimentAAV Tropism project
Experiment ConditionAAV3b males
Assay Description: Cre activity was assessed by image quantification
Tissue Measured:eye
Measurement Type:Editing Efficiency
Record ID15000001740

Editor
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAV3b-ZsGreen-Cre;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Sitewhole body
Dosage1E12 GC/mouse
Injection Frequencyonce
Injection Rate
Injection Volume150 microliters
Days post injection28 days
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 4
 
Replicate Result (Editing Efficiency)
Measurement Units: % cells tdTomato positive
Mean 0.08 
1 0.19 
2
3 0.13 
4
Measurement Units: GC/ug DNA
Mean 151.67 
1 495 
2 122 
3 241 
4 15 
5 20 
6 17 
  
AAV3b eye
AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging