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ExperimentAAV Tropism project
Experiment ConditionAAV3b males
Assay Description: Cre activity was assessed by image quantification
Tissue Measured:spleen
Measurement Type:Editing Efficiency
Record ID15000001751

Editor
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAV3b-ZsGreen-Cre;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Sitewhole body
Dosage1E12 GC/mouse
Injection Frequencyonce
Injection Rate
Injection Volume150 microliters
Days post injection28 days
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 4
 
Replicate Result (Editing Efficiency)
Measurement Units: % cells tdTomato positive
Mean 0.11 
1 0.02 
2 0.16 
3 0.13 
4 0.12 
Measurement Units: GC/ug DNA
Mean 14727.33 
1 36523 
2 15257 
3 11037 
4 2896 
5 15974 
6 6677 
  
AAV3b spleen
AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging