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ExperimentAAV Tropism project
Experiment ConditionAAV4 males
Assay Description: Cre activity was assessed by image quantification
Tissue Measured:brain
Measurement Type:Editing Efficiency
Record ID15000001778

Editor
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAV4-ZsGreen-Cre;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Sitewhole body
Dosage1E12 GC/mouse
Injection Frequencyonce
Injection Rate
Injection Volume150 microliters
Days post injection28 days
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 4
 
Replicate Result (Editing Efficiency)
Measurement Units: % cells tdTomato positive
Mean 3.95 
1 5.3 
2 4.17 
3 4.02 
4 2.33 
Measurement Units: GC/ug DNA
Mean 55825.33 
1 42250 
2 28520 
3 14265 
4 233 
5 86 
6 249598 
  
AAV4 brain
AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging