Experiment AAV Tropism project Experiment Condition AAV4 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: small intestine Measurement Type: Editing Efficiency Record ID 15000001790 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV4-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site whole body Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 7.55  1 4.36  2 8.5  3 10.39  4 6.93  Measurement Units: GC/ug DNA Mean 6745.83  1 5746  2 3489  3 13035  4 21  5 23  6 18161

AAV4 small intestine AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging