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ExperimentAAV Tropism project
Experiment ConditionAAV4 females
Assay Description: Cre activity was assessed by image quantification
Tissue Measured:thymus
Measurement Type:Editing Efficiency
Record ID15000001813

Editor
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAV4-ZsGreen-Cre;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Sitewhole body
Dosage1E12 GC/mouse
Injection Frequencyonce
Injection Rate
Injection Volume150 microliters
Days post injection28 days
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 4
 
Replicate Result (Editing Efficiency)
Measurement Units: % cells tdTomato positive
Mean 0.85 
1 0.41 
2 0.3 
3 1.46 
4 1.23 
Measurement Units: GC/ug DNA
Mean 471.33 
1
2 433 
3
4 739 
5 620 
6 1028 
  
AAV4 thymus
AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging