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ExperimentAAV Tropism project
Experiment ConditionAAV5 males
Assay Description: Cre activity was assessed by image quantification
Tissue Measured:testis
Measurement Type:Editing Efficiency
Record ID15000001834

Editor
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAV5-ZsGreen-Cre;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Sitewhole body
Dosage1E12 GC/mouse
Injection Frequencyonce
Injection Rate
Injection Volume150 microliters
Days post injection28 days
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 3
 
Replicate Result (Editing Efficiency)
Measurement Units: % cells tdTomato positive
Mean 2.63 
1 2.69 
2 4.36 
4 0.85 
Measurement Units: GC/ug DNA
Mean 2077.67 
1 471 
2 6223 
3 1643 
4 2979 
5 1007 
6 143 
  
AAV5 testis
AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging