Experiment AAV Tropism project Experiment Condition AAV5 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: diaphragm Measurement Type: Editing Efficiency Record ID 15000001839 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV5-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site whole body Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 34.8  1 53.44  2 18  3 20.7  4 47.04  Measurement Units: GC/ug DNA Mean 14778.67  1 5189  2 7380  3 47490  4 5867  5 18779  6 3967

AAV5 diaphragm AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging