Experiment AAV Tropism project Experiment Condition AAV5 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: small intestine Measurement Type: Editing Efficiency Record ID 15000001850 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV5-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site whole body Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 6.67  1 7.57  2 10.67  3 4.59  4 3.83  Measurement Units: GC/ug DNA Mean 808.67  1 81  2 1900  3 488  4 1440  5 573  6 370

AAV5 small intestine AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging