Experiment AAV Tropism project Experiment Condition AAV6 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: urinary bladder Measurement Type: Editing Efficiency Record ID 15000001857 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV6-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site whole body Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 12.46  1 7.95  2 17.25  3 14.87  4 9.76  Measurement Units: GC/ug DNA Mean 4356.5  1 6034  2 10744  3 3546  4 1166  5 543  6 4106

AAV6 urinary bladder AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging