Experiment AAV Tropism project Experiment Condition AAV6 females Assay Description: Cre activity was assessed by image quantification Tissue Measured: diaphragm Measurement Type: Editing Efficiency Record ID 15000001879 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV6-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site whole body Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 83.6  1 77.28  2 90.93  3 84.28  4 81.9  Measurement Units: GC/ug DNA Mean 38716.17  1 47339  2 47717  3 96119  4 29657  5 10791  6 674

AAV6 diaphragm AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging