Experiment AAV Tropism project Experiment Condition AAV9 males Assay Description: Cre activity was assessed by image quantification Tissue Measured: thymus Measurement Type: Editing Efficiency Record ID 15000001993 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV9-ZsGreen-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site whole body Dosage 1E12 GC/mouse Injection Frequency once Injection Rate Injection Volume 150 microliters Days post injection 28 days Editor Format Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % cells tdTomato positive Mean 7.03  1 2.07  2 9  3 8.17  4 8.87  Measurement Units: GC/ug DNA Mean 5558.6  1 896  2 785  3 2261  4 23159  5 692

AAV9 thymus AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging