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ExperimentAAV Tropism project
Experiment ConditionAAV9 females
Assay Description: Cre activity was assessed by image quantification
Tissue Measured:gastrocnemius
Measurement Type:Editing Efficiency
Record ID15000002001

Editor
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAV9-ZsGreen-Cre;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Sitewhole body
Dosage1E12 GC/mouse
Injection Frequencyonce
Injection Rate
Injection Volume150 microliters
Days post injection28 days
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 4
 
Replicate Result (Editing Efficiency)
Measurement Units: % cells tdTomato positive
Mean 87.75 
1 86.33 
2 93.62 
3 91.62 
4 79.43 
Measurement Units: GC/ug DNA
Mean 226646.83 
1 629104 
2 86227 
3 468050 
4 173207 
5 294 
6 2999 
  
AAV9 gastrocnemius
AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging