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ExperimentAAV Tropism project
Experiment ConditionAAVrh8 males
Assay Description: Cre activity was assessed by image quantification
Tissue Measured:diaphragm
Measurement Type:Editing Efficiency
Record ID15000002019

Editor
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAVrh8-ZsGreen-Cre;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Sitewhole body
Dosage1E12 GC/mouse
Injection Frequencyonce
Injection Rate
Injection Volume150 microliters
Days post injection28 days
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 4
 
Replicate Result (Editing Efficiency)
Measurement Units: % cells tdTomato positive
Mean 84.43 
1 71.83 
2 90.49 
3 90.51 
4 84.89 
Measurement Units: GC/ug DNA
Mean 275869.17 
1 544217 
2 133590 
3 116333 
4 652448 
5 132443 
6 76184 
  
AAVrh8 diaphragm
AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging