Contact SCGE




SCGE Consortium Home   |  About SCGE Toolkit   |  Contact Us   |  Citing the Toolkit   |  License  
ExperimentAAV Tropism project
Experiment ConditionAAVrh8 females
Assay Description: Cre activity was assessed by image quantification
Tissue Measured:diaphragm
Measurement Type:Editing Efficiency
Record ID15000002039

Editor
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAVrh8-ZsGreen-Cre;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Sitewhole body
Dosage1E12 GC/mouse
Injection Frequencyonce
Injection Rate
Injection Volume150 microliters
Days post injection28 days
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 4
 
Replicate Result (Editing Efficiency)
Measurement Units: % cells tdTomato positive
Mean 87.39 
1 77.48 
2 87.76 
3 92.86 
4 91.46 
Measurement Units: GC/ug DNA
Mean 490859.5 
1 38833 
2 82101 
3 25346 
4 136779 
5 1777252 
6 884846 
  
AAVrh8 diaphragm
AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging