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ExperimentAAV Tropism project
Experiment ConditionAAVrh74 females
Assay Description: Cre activity was assessed by image quantification
Tissue Measured:liver
Measurement Type:Editing Efficiency
Record ID15000002125

Editor
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAVrh74-ZsGreen-Cre;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Sitewhole body
Dosage1E12 GC/mouse
Injection Frequencyonce
Injection Rate
Injection Volume150 microliters
Days post injection28 days
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 4
 
Replicate Result (Editing Efficiency)
Measurement Units: % cells tdTomato positive
Mean 99.25 
1 99.11 
2 99.47 
3 99.19 
4 99.23 
Measurement Units: GC/ug DNA
Mean 36429703.67 
1 24422315 
2 35227334 
3 40243368 
4 39959321 
5 46233727 
6 32492157 
  
AAVrh74 liver
AAV serotypes delivering Cre recombinase were tested by intravenous delivery into Ai9 mice and chacterized by imaging