Experiment Podocyte-specific gene editing in human kidney organoids Experiment Condition RNP + MTA Assay Description: Calculated Editing efficiency by counting fluorescent cells (TdTomato) compared to Wilms Tumor-1 (WT1) stained cells. NOTE: Each data point represents one organoid. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000003588 Editor SpyCas9-ProteinG-3xNLS Delivery System endosomolytic peptide and anti-podocyte targeting antibody Delivery System Subtype Amphiphilic peptide Guide sg298 Vector Model Species human Model Name human kidney organoid with Ai9 (tdTomato) fluorescence-on reporter Application Method co-incubation Application Site Dosage Injection Frequency Injection Rate Injection Volume Days post injection 7 days Editor Format RNP+MTA Antidote Id Antidote Description Antibody RRID RRID:AB_2043201 Antibody Other ID Abcam (ab89901) Antibody Description anti-Wilms Tumor-1 (WT1) Measured Values Samples Size: 16   Replicate Result (Editing Efficiency) Measurement Units: WT1+tdTomato+/total tdTomato+ (%) Mean 75.44  1 66.6666667  2 63.6363636  3 66.6666667  4 60  5 80  6 75  7 77.7777778  8 71.4285714  9 80.7692308  10 50  11 66.6666667  12 88.8888889  13 81.8181818  14 77.7777778  15 100  16 100