SCGE ID11000000169
SymbolSpyCas9-ProteinG-3xNLS
DescriptionEngineered SpCas9 with protein G to bind immunoglobulins which binds to Fc subunit of antibodies. Has three different nuclear localization signals (NLS) located at both, the N-term and C-term.
SpeciesS. pyogenes
TypeCas
SubtypeCas9

ActivityDouble strand cleavage
Substratedouble stranded DNA
DSB Cleavage Typeblunt
PAM3' NGG

SourceRoss Wilson Lab


This Genome Editor: SpyCas9-ProteinG-3xNLS is being used ...
Project Initiative Contact PI
Collaborative Opportunity Fund Benjamin S Freedman, PhD Ross C. Wilson, PhD
(University of Washington)
NIH Report