RNP-NC-tri(GalNAc) | SCGE Toolkit

SCGE ID	12000000228
Name	RNP-NC-tri(GalNAc)
Description	The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and a guide RNA. This nanoparticle has a surface modification of hepatocyte-specific targeting ligand triGalNAc to enhance the internalization of NCs by liver cells.
Type	Nanoparticle
Subtype	polymer-RNP nanocapsule

Source	Gong Lab

Nanoparticle Size	34 nm
Zeta Potential	-3 mV

This Delivery System: RNP-NC-tri(GalNAc) is being used ...

Project	Initiative	Contact PI
Comparing the efficiency and cellular impact of non-viral, hepatocyte-targeted delivery vehicles with viral-mediated gene delivery in a microphysiological liver on chip platform Experiments Testing different ratios of Lipofectamine-RNPs after 24 hours for determination of positive control Quantification of transfection efficiency by flow cytometry with varying dosages of nanoparticles encapsulated with Cas9/sgRNA RNP on liver-on-chip model system Imaging quantification of transfection efficiency with varying dosages of nanoparticles encapsulated with Cas9/sgRNA RNP on the liver-on-chip model system	Collaborative Opportunity Fund	Ross C. Wilson, PhD Samira Kiani, PhD Shaoqin (Sarah) Gong, PhD (University of Pittsburgh)

Delivery System: RNP-NC-tri(GalNAc)