Genome Editor: Alt-R™ S.p. HiFi Cas9 Nuclease V3
Summary
| SCGE ID | 11000000174 |
| Symbol | Alt-R™ S.p. HiFi Cas9 Nuclease V3 |
| Description | Recombinant S. pyogenes Cas9 nuclease, purified from an E. coli strain expressing the nuclease. Contains nuclear localization sequence (NLS) and C-terminal 6-His tag. Provided in solution at 10 µg/µL. 100 µg of Cas9 nuclease = 610 pmol. Has improved specificity based on reduced off-target effects. |
| Species | Streptococcus pyogenes |
| Type | Cas Nuclease |
| Subtype | Cas9 |
| Activity | Double strand cleavage |
| Substrate | double stranded DNA |
| DSB Cleavage Type | Blunt |
| PAM | 3' NGG |
| Source | Integrated DNA Technologies (IDT) |
Projects & Experiments
This Genome Editor: Alt-R™ S.p. HiFi Cas9 Nuclease V3 is being used ...| Project | Initiative | Contact PI | |
|---|---|---|---|
| Biological Effects |
Benjamin S Freedman, PhD
(University of Washington) |
|