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ExperimentTesting virus region 4 (VR4) mutant cross-species compatible Adeno Associated Viruses (ccAAVs) in mice.
Experiment ConditionAAVcc47-mCherry
Assay Description: Native fluorescence was quantified using Image J. For each animal, 6 images were taken from 2 sections and used to calculate corrected total cell fluorescence using the formula: Integrated Density – (Area of selected image X Mean fluorescence of background readings).
Tissue Measured:heart
Measurement Type:Delivery Efficiency
Record ID15000000345

Editor
Delivery System
Delivery System Subtype
Guide
VectorAAVcc47-mCherry;

Model SpeciesMouse
Model NameC57BL/6

Application Methodintravenous
Application Site
Dosage5e13 vg/kg
Injection Frequency1X
Injection Rate
Injection Volume200 microliters
Days post injection4 weeks
Editor FormatAAV vector encoded
Antidote Id
Antidote Description

Measured Values
Samples Size: 3
 
Replicate Result (Delivery Efficiency)
Measurement Units: Corrected Total Fluorescence
Mean 6367289609.93 
1 9909939943 
2 6167627062.05556 
3 3024301824.72222 
  

Representative image of AAVcc47-mCherry delivery in heart