Experiment Testing AAV5 for activation of tdTomato in mouse airway Experiment Condition AAV-SpCas9 Assay Description: Two AAVs encoding sgRNAs and SpCas9 were introduced by intratracheal injection into Ai9 mice. After 4 weeks, lung tissue was harvested and prepared by sectioning and immunohistochemical staining for GFP. Delivery efficency was calculated by counting GFP+ cells compared to DAPI stained cells. Tissue Measured: epithelium of main bronchus Cell Type : epithelial cell of lung Measurement Type: Delivery Efficiency Record ID 15000001635 Editor SpCas9 Delivery System Delivery System Subtype Guide Vector AAV.pU1a-SpCas9; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method inhalation Application Site airway epithelium Dosage 6e10 gc/each AAV per mouse by intratracheal injection Injection Frequency once Injection Rate n/a Injection Volume 40 microliters Days post injection 4 weeks Editor Format AAV vector encoded Antidote Id Antidote Description Antibody RRID Antibody Other ID Cell Signaling Technology Cat# 2956 Antibody Description GFP (D5.1) XP Rabbit mAb antibody Antibody RRID Antibody Other ID Rockland Cat# 600-401-379 Antibody Description Anti-RFP (RABBIT) Antibody Measured Values Samples Size: 5   Replicate Result (Delivery Efficiency) Measurement Units: % cells Mean 0.0  1 0  2 0  3 0  4 0  5 0