Development System
AAVS1_site_13 | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
A novel human T cell platform to define biological adverse effects of genome editing |
AAVS1_site_13 |
Calculated editing efficiency by targeted amplicon deep sequencing |
|
Editing Efficiency |
15000000433 |
|
SpCas9 |
P3 Nucleofection Kit |
Commercial Reagent |
AAVS1_site_13 |
|
|
Human |
CD4/CD8 Human Primary T cell |
|
nucleofection |
|
75 pmol Cas9 protein; 3-fold molar excess of sgRNA |
|
|
|
|
ribonucleoprotein (RNP) |
|
|
|
Measured Values |
Samples Size: | 3 |
|
Mean |
41.18  |
1 |
41.698918150234896  |
2 |
41.7140358548317  |
3 |
40.1194747062502  |
| |
|
Publication Title |
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI |