Experiment A novel human T cell platform to define biological adverse effects of genome editing Experiment Condition PDCD1_site_02 Assay Description: Calculated editing efficiency by targeted amplicon deep sequencing Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000497 Editor SpCas9 Delivery System P3 Nucleofection Kit Delivery System Subtype Commercial Reagent Guide PDCD1_site_02 Vector Model Species Human Model Name CD4/CD8 Human Primary T cell Application Method nucleofection Application Site Dosage 75 pmol Cas9 protein; 3-fold molar excess of sgRNA Injection Frequency Injection Rate Injection Volume Days post injection Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % of reads Mean 94.33  1 94.55063033753561  2 94.1460126516644  3 94.2970045858132