Experiment A novel human T cell platform to define biological adverse effects of genome editing Experiment Condition PDCD1_site_14 Assay Description: Calculated editing efficiency by targeted amplicon deep sequencing Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000509 Editor SpCas9 Delivery System P3 Nucleofection Kit Delivery System Subtype Commercial Reagent Guide PDCD1_site_14 Vector Model Species Human Model Name CD4/CD8 Human Primary T cell Application Method nucleofection Application Site Dosage 75 pmol Cas9 protein; 3-fold molar excess of sgRNA Injection Frequency Injection Rate Injection Volume Days post injection Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % of reads Mean 12.52  1 12.6249828790577  2 12.2567264255476  3 12.6877064423904