Experiment A novel human T cell platform to define biological adverse effects of genome editing Experiment Condition PTPN6_site_03 Assay Description: Calculated editing efficiency by targeted amplicon deep sequencing Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000517 Editor SpCas9 Delivery System P3 Nucleofection Kit Delivery System Subtype Commercial Reagent Guide PTPN6_site_03 Vector Model Species Human Model Name CD4/CD8 Human Primary T cell Application Method nucleofection Application Site Dosage 75 pmol Cas9 protein; 3-fold molar excess of sgRNA Injection Frequency Injection Rate Injection Volume Days post injection Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % of reads Mean 78.58  1 79.1606210098665  2 78.1767701558473  3 78.3928732385903