Experiment Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells Experiment Condition STS119 (TLR-MCV1b) (20 pmol RNP) Assay Description: Calculated editing efficiency by flow cytometry analysis of RFP expression. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000549 Editor SpyCas9-3xNLS Delivery System Delivery System Subtype Guide STS119 (TLR-MCV1b; Sp_t2:Sp_c20_TLR_MCV1b) Vector Model Species Human Model Name HEK-293T-disrupted_GFP with MCV-mcherry-Puro Application Method electroporation Application Site Dosage 20 pmol RNP Injection Frequency Injection Rate Injection Volume Days post injection 48-72 hours Editor Format protein, RNA Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: % of RFP+ cells Mean 22.3  1 23  2 21.6