Experiment Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells Experiment Condition STS120 (TLR1) (3 pmol RNP) Assay Description: Calculated editing efficiency by flow cytometry analysis of RFP. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000552 Editor SpyCas9-3xNLS Delivery System Delivery System Subtype Guide STS120 (TLR1; Sp_t2:Sp_c20_TLR1) Vector Model Species Human Model Name HEK-293T-disrupted_GFP-mcherry-Puro Sample Age 4-12 Sample Sex Female Application Method electroporation Application Site Dosage 3 pmol RNP Injection Frequency Injection Rate Injection Volume Days post injection 48-72 hours Editor Format protein, RNA Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % of RFP+ cells Mean 25.0  1 22  2 26  3 27