Experiment Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells Experiment Condition STS134 (PCSK9b) (5 pmol RNP) Assay Description: Calculated editing efficiency by PCR amplification and sanger sequencing analysis. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000555 Editor SpyCas9-3xNLS Delivery System Delivery System Subtype Guide STS134 (PCSK9b; Sp_t2:Sp_c20_PCSK9b) Vector Model Species Mouse Model Name Hepa1-6 Application Method electroporation Application Site Dosage 5 pmol RNP Injection Frequency Injection Rate Injection Volume Days post injection 72 hours Editor Format protein, RNA Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: % of Lesion Mean 96.0  1 96  2 96