Experiment Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells Experiment Condition STS205 (BACE1) (5 pmol RNP) Assay Description: Calculated editing efficiency by PCR amplification and sanger sequencing analysis. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000561 Editor SpyCas9-3xNLS Delivery System Delivery System Subtype Guide STS205 (BACE1; Sp_t41:Sp_c20_Bace1) Vector Model Species Mouse Model Name Neuro 2A Application Method electroporation Application Site Dosage 5 pmol RNP Injection Frequency Injection Rate Injection Volume Days post injection 72 hours Editor Format protein, RNA Antidote Id Antidote Description Measured Values Samples Size: 1   Replicate Result (Editing Efficiency) Measurement Units: % of Lesion Mean 28.0  1 28.0