Development System
cc47-SaCas9, Female | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
Independent validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. |
cc47-SaCas9, Female |
Editing efficiency calculated by counting fluorescent cells (RFP) compared to DAPI stained nuclei |
heart |
cardiac muscle cell |
Editing Efficiency |
15000001270 |
|
SauCas9 |
|
|
Ai9 SaCas9 Guide A; Ai9 SaCas9 Guide B |
AAVcc47-SaCas9-Ai9; |
|
Mouse |
B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J |
|
intravenous (tail vein) |
liver and heart |
1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) |
once |
|
200 microliters |
4 weeks |
AAV vector encoded |
|
|
|
Measured Values |
Samples Size: | 5 |
|
Mean |
4.83  |
1 |
4.66  |
2 |
5.86  |
3 |
2.9  |
4 |
5.11  |
5 |
5.61  |
| |
|