Validation Experiment: Independent validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing.
PI: Jason D Heaney, PhD
Description: Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues.
Editing Assay:
- Fluorescent imaging for tdTomato+ cells
- Editing efficiency calculated by counting fluorescent cells (RFP) compared to DAPI stained nuclei
Parent Project: BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - Asokan Validation (Asokan, Aravind)
Download: Submitted files
Original Experiment/s that are being validated: