Validation Experiment: Independent validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing.
PI: Jason D Heaney, PhD
Description: Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues.
Editing Assay:
- Fluorescent imaging for tdTomato+ cells
- Editing efficiency calculated by counting fluorescent cells (RFP) compared to DAPI stained nuclei
Parent Project: BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - Asokan Validation (Asokan, Aravind)
Download: Submitted files
Original Experiment/s that are being validated:
Analyze Data Sets Available for this Experiment
Connective Tissue
| Brown adipose tissue |
| Gonadal fat pad |
| Subcutaneous adipose tissue |
| Uterus |
| Epididymis |
| Testis |
| Ovary |
| Kidney |
| Pancreas |
| Spleen |
| Duodenum |
| Colon |
| Ileum |
| Jejunum |
| Stomach |
| Liver (hepatocyte) (TARGET) |
| Lung |
| Heart (cardiac muscle cell) (TARGET) |
| Diaphragm |
| Extensor digitorum longus |
| Gastrocnemius |
| Soleus muscle |
| Transversus abdominis muscle |
| Brain |
| Eye |
Organ System Overview
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