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  Tissues
Connective Tissue
  • Brown adipose tissue
  • Gonadal fat pad
  • Subcutaneous adipose tissue
Reproductive
  • Uterus
  • Epididymis
  • Testis
  • Ovary
Renal/Urinary
  • Kidney
Endocrine
  • Pancreas
Haemolymphoid
  • Spleen
Gastrointestinal
  • Duodenum
  • Colon
  • Ileum
  • Jejunum
  • Stomach
Liver and Biliary
  • Liver (hepatocyte)  (TARGET)
Respiratory
  • Lung
Cardiovascular
  • Heart (cardiac muscle cell)  (TARGET)
Musculoskeletal
  • Diaphragm
  • Extensor digitorum longus
  • Gastrocnemius
  • Soleus muscle
  • Transversus abdominis muscle
Nervous
  • Brain
Sensory
  • Eye
  Cell Types
 cardiac muscle cell
 hepatocyte
  Editors
 Cre recombinase
 SauCas9
Guide Target Locus
 Ai9/Ai14 reporter transgene
  Guides
 Ai9 SaCas9 Guide A
 Ai9 SaCas9 Guide B
Models
 Ai9 mouse (BCM)
  Sex
 Female
 Male
  Vectors
 AAVcc47-Cre
 AAVcc47-SaCas9-Ai9
Select experimental variable to highlight records on the chart: 
 

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Results

Independent_validation_for_Asokan_Delivery_Team:_Evolving_High_Potency_AAV_Vectors_for_Neuromuscular_Genome_Editing.
Editing Efficiency
Record Id Condition Tissue Cell Type Sex Editor Model Target Locus Guide Vector Dosage Injection Frequency Editing Efficiency Signal Editing Efficiency % of cells Image
15000001297 Saline Neg control, Male heart (TARGET) cardiac muscle cell Male Ai9 mouse (BCM) once
15000001298 Saline Neg control, Female liver (TARGET) hepatocyte Female Ai9 mouse (BCM) once
15000001252 cc47-SaCas9, Male duodenum Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001291 cc47-SaCas9, Female uterus Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001293 cc47-Cre Pos control, Male heart (TARGET) cardiac muscle cell Male Cre recombinase Ai9 mouse (BCM) AAVcc47-Cre 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001294 cc47-Cre Pos control, Female liver (TARGET) hepatocyte Female Cre recombinase Ai9 mouse (BCM) AAVcc47-Cre 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001246 cc47-SaCas9, Male liver (TARGET) hepatocyte Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001247 cc47-SaCas9, Male heart (TARGET) cardiac muscle cell Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001248 cc47-SaCas9, Male brain Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001249 cc47-SaCas9, Male brown adipose tissue Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001250 cc47-SaCas9, Male colon Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001251 cc47-SaCas9, Male diaphragm Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001253 cc47-SaCas9, Male epididymis Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001254 cc47-SaCas9, Male extensor digitorum longus Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001255 cc47-SaCas9, Male eye Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001256 cc47-SaCas9, Male gastrocnemius Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001257 cc47-SaCas9, Male ileum Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001258 cc47-SaCas9, Male jejunum Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001259 cc47-SaCas9, Male kidney Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001260 cc47-SaCas9, Male lung Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001261 cc47-SaCas9, Male pancreas Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001262 cc47-SaCas9, Male gonadal fat pad Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001263 cc47-SaCas9, Male soleus muscle Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001264 cc47-SaCas9, Male spleen Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001265 cc47-SaCas9, Male stomach Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001266 cc47-SaCas9, Male subcutaneous adipose tissue Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001267 cc47-SaCas9, Male testis Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001268 cc47-SaCas9, Male transversus abdominis muscle Male SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001269 cc47-SaCas9, Female liver (TARGET) hepatocyte Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001270 cc47-SaCas9, Female heart (TARGET) cardiac muscle cell Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001271 cc47-SaCas9, Female brain Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001272 cc47-SaCas9, Female brown adipose tissue Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001273 cc47-SaCas9, Female colon Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001274 cc47-SaCas9, Female diaphragm Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001275 cc47-SaCas9, Female duodenum Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001276 cc47-SaCas9, Female extensor digitorum longus Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001277 cc47-SaCas9, Female eye Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001278 cc47-SaCas9, Female gastrocnemius Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001279 cc47-SaCas9, Female ileum Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001280 cc47-SaCas9, Female jejunum Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001281 cc47-SaCas9, Female kidney Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001282 cc47-SaCas9, Female lung Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001283 cc47-SaCas9, Female pancreas Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001284 cc47-SaCas9, Female gonadal fat pad Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001285 cc47-SaCas9, Female soleus muscle Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001286 cc47-SaCas9, Female spleen Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001287 cc47-SaCas9, Female stomach Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001288 cc47-SaCas9, Female subcutaneous adipose tissue Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001289 cc47-SaCas9, Female transversus abdominis muscle Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001290 cc47-SaCas9, Female ovary Female SauCas9 Ai9 mouse (BCM) Ai9/Ai14 reporter transgene Ai9 SaCas9 Guide AAi9 SaCas9 Guide B AAVcc47-SaCas9-Ai9 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001292 cc47-Cre Pos control, Male liver (TARGET) hepatocyte Male Cre recombinase Ai9 mouse (BCM) AAVcc47-Cre 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001295 cc47-Cre Pos control, Female heart (TARGET) cardiac muscle cell Female Cre recombinase Ai9 mouse (BCM) AAVcc47-Cre 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) once
15000001296 Saline Neg control, Male liver (TARGET) hepatocyte Male Ai9 mouse (BCM) once
15000001299 Saline Neg control, Female heart (TARGET) cardiac muscle cell Female Ai9 mouse (BCM) once

Title Description File Download SCGE ID
Heaney_SATC Tissue Processing, Imaging and Analysis Procedure for tissue preparation, imaging and analysis. Heaney_SATC_Tissue_Processing_Imaging_and_Analysis.pdf 21000000025