Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) |
AAVcc47, Male |
Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells. |
heart |
Editing Efficiency |
15000001010 |
|
SaCas9-Lagor |
|
|
|
AAVcc47-Ai9-sgRNA1-CB-SaCas9; AAVcc47-Ai9-sgRNA2-CB-SaCas9; |
|
Mouse |
B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J |
|
intravenous |
liver and heart |
1e12vg each vector mixed 1:1 |
once |
|
200uL |
4 weeks |
SaCas9 protein and sgRNAs expressed from AAV |
|
|
|
Measured Values |
Samples Size: | 3 |
|
Mean |
1.07  |
1 |
1.11170476910674  |
2 |
0.618006255986144  |
3 |
1.48503157057337  |