Experiment Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) Experiment Condition AAVcc47, Female Assay Description: Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells. Tissue Measured: heart Measurement Type: Editing Efficiency Record ID 15000001012 Editor SaCas9-Lagor Delivery System Delivery System Subtype Guide Vector AAVcc47-Ai9-sgRNA1-CB-SaCas9; AAVcc47-Ai9-sgRNA2-CB-SaCas9; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site liver and heart Dosage 1e12vg each vector mixed 1:1 Injection Frequency once Injection Rate Injection Volume 200uL Days post injection 4 weeks Editor Format SaCas9 protein and sgRNAs expressed from AAV Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: % of cells Mean 1.47  1 1.6157352166944  2 1.32183822066003