Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) |
AAV9, Male |
Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells. |
liver |
Editing Efficiency |
15000001015 |
|
SaCas9-Lagor |
|
|
|
AAV9-Ai9-sgRNA1 + sgRNA2; AAV9-CMV-SaCas9; |
|
Mouse |
B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J |
|
intravenous |
liver and heart |
1.5e12vg each vector mixed 1:1 |
once |
|
200uL |
4 weeks |
SaCas9 protein and sgRNAs expressed from AAV |
|
|
|
Measured Values |
Samples Size: | 2 |
|
Mean |
7.42  |
1 |
7.494040184763  |
2 |
7.34243307861485  |