| Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) |
| AAVcc47, Male |
| Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells. |
| heart |
| Editing Efficiency |
| 15000001020 |
|
| SaCas9-Lagor |
|
|
|
| AAVcc47-Ai9-sgRNA1 + sgRNA2; AAVcc47-CMV-SaCas9; |
|
| Mouse |
| B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J |
|
| intravenous |
| liver and heart |
| 1.5e12vg each vector mixed 1:1 |
| once |
|
| 200uL |
| 4 weeks |
| SaCas9 protein and sgRNAs expressed from AAV |
|
|
|
| Measured Values |
| Samples Size: | 3 |
| |
| Mean |
1.45 |
| 1 |
1.28400230140656 |
| 2 |
1.44614756063939 |
| 3 |
1.62578049621291 |