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ExperimentComparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
Experiment ConditionMock-Neg Control
Assay Description: Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells.
Tissue Measured:liver
Measurement Type:Editing Efficiency
Record ID15000001031

Editor
Delivery System
Delivery System Subtype
Guide
Vector

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Siteliver and heart
Dosage
Injection Frequency
Injection Rate
Injection Volume
Days post injection4 weeks
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 2
 
Replicate Result (Editing Efficiency)
Measurement Units: % of cells
Mean 0.01 
1 0.018053927 
  

Publication Title
Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing. NCBI
Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing. NCBI