Experiment Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector. Experiment Condition AAVcc47, Male Assay Description: Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells. Tissue Measured: heart Measurement Type: Editing Efficiency Record ID 15000001038 Editor SaCas9-Lagor Delivery System Delivery System Subtype Guide Vector AAV9-CMV-SaCas9; AAVcc47_pTR_self comp 2xU6-Ai9 guides; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site liver and heart Dosage 2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector Injection Frequency once Injection Rate Injection Volume 200uL Days post injection 4 weeks Editor Format SaCas9 protein and sgRNAs expressed from AAV Antidote Id Antidote Description Measured Values Samples Size: 1   Replicate Result (Editing Efficiency) Measurement Units: % of cells Mean 2.86  1 2.855849