AAVcc47_pTR_self comp 2xU6-Ai9 guides | SCGE Toolkit

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Summary Projects & Experiments

SCGE ID	14000000052
Name	AAVcc47_pTR_self comp 2xU6-Ai9 guides
Description	AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene
Type	Viral Vector
Subtype	AAV

Source	Asokan Lab

Genome Serotype	AAV2
Capsid Serotype	AAV9
Capsid Variant	452-GVSLGGG-458
Stock/Catalog/RRID	AAVcc47_pTR_self comp 2xU6-Ai9 guides

Annotated Map	pLH9-GVSLGGG.gb
Titer Method	qPCR

Publication Title
Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing. NCBI

This Vector/Format: AAVcc47_pTR_self comp 2xU6-Ai9 guides is being used ...

Project	Initiative	Contact PI
Evolving High Potency AAV Vectors for Neuromuscular Genome Editing Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.	Delivery Systems	Aravind Asokan, PhD (Duke University)