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ExperimentComparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
Experiment ConditionAAVcc47, Female
Assay Description: Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells.
Tissue Measured:liver
Measurement Type:Editing Efficiency
Record ID15000001039

EditorSaCas9-Lagor
Delivery System
Delivery System Subtype
Guide
VectorAAV9-CMV-SaCas9; AAVcc47_pTR_self comp 2xU6-Ai9 guides;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Siteliver and heart
Dosage2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector
Injection Frequencyonce
Injection Rate
Injection Volume200uL
Days post injection4 weeks
Editor FormatSaCas9 protein and sgRNAs expressed from AAV
Antidote Id
Antidote Description

Measured Values
Samples Size: 3
 
Replicate Result (Editing Efficiency)
Measurement Units: % of cells
Mean 5.96 
1 6.13115548272134 
2 5.67915729687348 
3 6.05833220265549