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ExperimentIndependent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice
Experiment ConditionssAAV5-spCas9 + ssAAV5-sgA+sgB-U1A.GFP, Ai9 - SauSpyCas9, male
Assay Description: Editing efficiency is calculated by identifying airways in lungs sections by shape and DAPI-stained nuclei density. tdTomato-positive cells and total nuclei are counted in each airway. Airways are divided into small (<50000 square microns) and large (>50000 square microns)
Tissue Measured:epithelium of main bronchus
Cell Type :respiratory epithelial cell
Measurement Type:Editing Efficiency
Record ID15000001049

EditorSpCas9
Delivery System
Delivery System Subtype
GuideBCM_ssAAV5-Sp_sgA
VectorssAAV5-sgA+sgB-U1A.GFP; ssAAV5-spCas9;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor^em1(CAG-tdTomato)JaHe

Application MethodIntratracheal Intubation
Application Sitelung airway epithelium
Dosage1.7E11 viral genomes
Injection Frequencyonce
Injection Rate
Injection Volume40 microliters
Days post injection4 weeks
Editor Formatvirus in sterile saline
Antidote Id
Antidote Description

Measured Values
Samples Size: 1
 
Replicate Result (Editing Efficiency)
Measurement Units: % of cells
Mean 17.6 
1 17.6 
  

Title Description File Download SCGE ID
Heaney_SATC Tissue Processing, Imaging and Analysis Procedure for tissue preparation, imaging and analysis. Heaney_SATC_Tissue_Processing_Imaging_and_Analysis.pdf 21000000025
Heaney-SATC_Gao-Validation_Intratracheal Delivery of AAV in Mice Procedure for Intratracheal (IT) delivery of AAV in mouse lung. Heaney-SATC_Gao-Validation_Intratracheal_Delivery_of_AAV_in_Mice.pdf 21000000023