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ExperimentIndependent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice
Experiment Conditionsaline Neg control, male
Assay Description: Fluorescent imaging for tdTomato+ cells
Tissue Measured:soleus muscle
Measurement Type:Editing Efficiency
Record ID15000001102

Editor
Delivery System
Delivery System Subtype
Guide
Vector

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor^em1(CAG-tdTomato)JaHe

Application Methodintratracheal intubation
Application Sitelung airway epithelium
Dosage
Injection Frequencyonce
Injection Rate
Injection Volume40 microliters
Days post injection4 weeks
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 3
 
Replicate Result (Editing Efficiency)
Measurement Units: Signal
Mean 3 of 3 no red cells 
1 No red cells 
2 No red cells 
3 No red cells 
  

Title Description File Download SCGE ID
Heaney_SATC Tissue Processing, Imaging and Analysis Procedure for tissue preparation, imaging and analysis. Heaney_SATC_Tissue_Processing_Imaging_and_Analysis.pdf 21000000025
Heaney-SATC_Gao-Validation_Intratracheal Delivery of AAV in Mice Procedure for Intratracheal (IT) delivery of AAV in mouse lung. Heaney-SATC_Gao-Validation_Intratracheal_Delivery_of_AAV_in_Mice.pdf 21000000023